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KMID : 0370220030470020093
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Yakhak Hoeji
2003 Volume.47 No. 2 p.93 ~ p.97
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Antioxidant Effect of Kombucha Broth Against Scenescence Induced Normal Human Diploid Fibroblasts with Oxygen Free Radicals
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이상은/Lee SE
최진석/이강훈/김국환/권영이/Choi JS/Lee KH/Kim KW/Kwon YE
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Abstract
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Kombucha fermentation broth has been used as a popular health beverage and an alternative therapy with prophylactic and therapeutic benefit. We tried to establish optimal cult ure conditions for Kombucha fermentation in milk and to investigate cytotoxicity and antioxidative enzyme activity of Kombucha broth against normal human fibroblasts. The optimal conditions of Kombuch culture were established to 30℃,20~23 hours by DPPH radical scavenging test. There were positive erects on cell growth while no cytotoxicity against primary normal human diploid fibroblasts was found. The activites of glutathione peroxide and catalase in the cells treated by hydrogen peroxide (1 mM) alone and by hydrogen peroxide with Kombucha broth (1 mg/ml) were significantly different (p<0.05). These results suggest that Kombucha broth could be developed as an antioxidant agent for a new cosmetic material.
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